Chloroquine transfection mechanism

Discussion in 'Hydroxychloroquine' started by Souldrinker, 12-Mar-2020.

  1. Kylo2V Moderator

    Chloroquine transfection mechanism


    Transfections allow for transient expression of a gene of interest in a target cell line and can be useful for short term studies of protein function. We specifically use this protocol with Lenti-X 293T cells, a cell line optimized for production of lentiviral vectors.

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    Transfection is the process of introducing nucleic acids into eukaryotic cells by nonviral methods. Using various chemical or physical methods, this gene transfer technology enables the study of gene function and protein expression in a cellular environment. Article On the Mechanism of Chloroquine Resistance. daily without chloroquine is not as abundant as a single one-time collection between 48-60 hours after initial transfection with chloroquine. Chloroquine is commonly used to study the role of endosomal acidification in cellular processes 2, 3, such as the signaling of intracellular TLRs. Moreover, Chloroquine inhibits autophagy as it raises the lysosomal pH, which leads to inhibition of both fusion of autophagosome with lysosome and lysosomal protein degradation 4. References. 1.

    Last Upload: June 10, 2016 Day 0: Seed Lenti-X 293T cells (this cell line is optimized for production of lentiviral vectors) Day 1 (pm): Transfect Cells Day 2 (am): 18h post transfection - Remove media, replace with fresh media Day 3 or more (am): Observe fluorescence, harvest cells, or perform your experiment *Pro-Tips* Different brands and lots of FBS can promote or inhibit transfection. This approach can be adapted for different cell lines and different transfection reagents.

    Chloroquine transfection mechanism

    Chloroquine - Wikipedia, Questions with answers in CHLOROQUINE Science topic

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  3. Transfection efficiency was affected by the concentration of lysosomotropic agents. The marked transfection enhancement was observed when the amount of sucrose increased from 5 to 500 mM and the optimum transfection efficiency was found at 500 mM in all cell lines tested Fig. 2 a–c. Transgene expression was found to be very low when the cells were transfected with plasmid alone.

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    Figure 1. Structure of chloroquine and some related synthetic compounds that are effective against chloroquine-resistant as well as chloroquine-sensitive P. binding depends on the stacking interactions between the 7-chloro-substituted quinoline ring and products of heme released from the digestion of host cell hemoglobin. Similarly, the transformation frequency of rat cells following polyoma DNA transfection was approximately 6-fold increased by chloroquine treatment. The effect of the compound was even more pronounced in transfections with linear forms of polyoma DNA, suggesting that chloroquine inhibits degradation of DNA absorbed by the cells. Chloroquine is an inhibitor of the lysosomal degradation of the DNA which is taken up by the cells, so as leelee said, transfection should have been successful, albeit at a slightly lower level than if you would have added the chloroquine. In my normal transfections, I never add chloroquine and get high efficiencies nevertheless.

     
  4. forb Guest

    Hydroxychloroquine is a quinoline medicine used to treat or prevent malaria, a disease caused by parasites that enter the body through the bite of a mosquito. Plaquenil, Plaquenil Sulfate, Quineprox. PLAQUENIL® HYDROXYCHLOROQUINE SULFATE TABLETS, USP Hydroxychloroquine Professional Patient Advice -
     
  5. Slajzer New Member

    Download PDF Many systemic medications may cause retinal toxicity. Hydroxychloroquine retinopathy Eye Hydroxychloroquine Plaquenil Toxicity and. PDF Multifocal ERG Guiding Therapy in a Case of.
     
  6. jssa New Member

    Fundus Autofluorescence Is Not the Best Early Screen for. Fundus Autofluorescence Is Not the Best Early Screen for Hydroxychloroquine Toxicity—Reply. These images were intended to remind ophthalmologists that.

    Recommendations on Screening for Chloroquine and.